TABLE 1.

Influence of the SCWP on in vitro self-assembly of the S-layer protein from B. stearothermophilus PV72/p2

Amt of SCWP (μg/mg of S-layer protein)a	Molar ratio (SCWP/S-layer subunits)	DA (%)b	% Coverage of poly-l-lysinecoated EM grids with monolayer patches
10	1:24	90	0
20	1:12	80	0
30	1:8	60	NDc
60	1:4	20	30–50
100	1:2.4	15	50–70
120	1:2	10	ND
150	1:1.6	10	>70
250	1:1	5	>90
600	1:0.4	5	<90

^aDifferent amounts of SCWP were added to the GHCl-extracted S-layer protein or to the fraction containing the soluble (monomeric and/or oligomeric) S-layer protein. 

^bThe DA was determined after 18 h of dialysis against 10 mM CaCl₂ at 20°C as described in the text. 

^cND, not determined.