TABLE 1.
Bacterial strains and vectors used
| Strain or vector | Genotype or description | Reference or source |
|---|---|---|
| L. sake strains | ||
| 23K | Wild-type isolate | 1 |
| RV4000 | 23K, arcA::pRV300, Erir | This work |
| RV4010 | 23K, arcB::pRV300, Erir | This work |
| RV4020 | 23K, arcC::pRV300, Erir | This work |
| RV4030 | 23K, arcD::pRV300, Erir | This work |
| BL13 | Type strain | CECT 906 |
| E. coli strains | ||
| XL1Blue | recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 (F′ proAB lacIqlacZΔM15 Tn10) | Stratagene |
| LE392 | F−hsdR574 (rK− mK−) supE44 supF58 lacY1 galK2 galT22 metB1 trpR55 | Promega |
| Cloning vectors | ||
| pACYC184 | Tcr Cmr | New England Biolabs |
| pBluescriptII SK+ | Amr | Stratagene |
| pT7Blue T-vector | Amr | Novagen |
| pRV300 | Erir from pAMβ1 | 13 |
| λEMBL3 | BamHI arms | Promega |
| λ-arc | λ arms ligated to the L. sake BL13 chromosomal fragment containing the arc genes | This work |
| pACYC-ER1 | pACYC184 containing EcoRI fragment 1 (see Fig. 2) | This work |
| pBS-H5 | pBluescript containing HindIII fragment 5 (see Fig. 2) | This work |
| pBS-H8 | pBluescript containing HindIII fragment 8 (see Fig. 2) | This work |
| pBS-N1 | pBluescript containing NheI fragment 1 (see Fig. 2) | This work |
| pBS-N2 | pBluescript containing NheI fragment 2 (see Fig. 2) | This work |