Table 4.
Identification and quantification of polyphenolic compounds present in L. angustifolia hydroalcoholic extracts.
| RF | LA Hydroalcoholic Extracts (mg/kg Plant) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| LA-1 | LA-2 | LA-3 | LA-4 | LA-5 | LA-6 | LA-7 | LA-8 | LA-9 | LA-10 | LA-11 | LA-12 | |
| TA | 28.0 ± 0.4 g | 34.5 ± 2.4 g | 49.5 ± 0.4 f | 50.4 ± 2.5 f | 83.1 ± 0.9 d | 91.8 ± 2.5 c | 111.5 ± 2.3 b | 122.6 ± 0.7 a | 56.1 ± 0.6 f | 51.1 ± 0.3 f | 74.7 ± 1.0 e | 69.3 ± 1.2 e |
| GA | ND | ND | 10 ± 0.0 g | 10.7 ± 0.2 g | 25.5 ± 0.0 c | 28.6 ± 0.9 b | 29.6 ± 0.2 b | 42.1 ± 0.1 a | 16.8 ± 0.9 e | 14.3 ± 0.4 f | 23.4 ± 0.2 d | 18.8 ± 0.1 e |
| PA | 3107.0 ± 17.0 k | 4350.2 ± 40.1 j | 4982.8 ± 48.9 i | 5648.7 ± 64.7 h | 9196.7 ± 32.1 d | 9857.3 ± 97.9 c | 10,223.0 ± 31.2 b | 11,025.0 ± 39.0 a | 7510.4 ± 21.2 f | 7202.9 ± 48.8 g | 7837.8 ± 85.7 e | 7793.2 ± 96.6 ef |
| CA | 47.1 ± 0.2 f | 65.9 ± 4.2 f | 96.5 ± 0.3 e | 103.5 ± 4.3 e | 154.8 ± 11.6 bc | 159.9 ± 0.5 bc | 172.4 ± 3.9 b | 221.7 ± 12.5 a | 117.5 ± 0.3 de | 105.5 ± 4.6 e | 137.0 ± 2.5 cd | 134.8 ± 0.5 cd |
| CF | 33.3 ± 1.2 g | 36.3 ± 1.2 fg | 41.9 ± 3.7 ef | 45.2 ± 2.0 de | 56.0 ± 0.9 bc | 60.5 ± 1.4 ab | 66.3 ± 0.3 a | 68.6 ± 0.9 a | 47.7 ± 1.2 ce | 46.5 ± 1.8 de | 55.1 ± 0.9 bc | 50.5 ± 1.9 cd |
| CH | ND | ND | ND | ND | 33.0 ± 3.8 b | 40.3 ± 0.9 b | 67.3 ± 2.1 a | 70.9 ± 9.4 a | ND | ND | ND | ND |
| SY | 1017.3 ± 11.6 i | 1059.0 ± 0.4 hi | 1180.8 ± 2.0 gh | 1214.5 ± 6.0 g | 3014.7 ± 2.0 c | 3181.2 ± 31.5 b | 3048.7 ± 38.2 c | 3847.3 ± 2.0 a | 1653.5 ± 10.8 e | 1426.2 ± 40.3 f | 1863.7 ± 49.6 d | 1663.7 ± 21.8 e |
| EP | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND |
| PC | 10.8 ± 0.3 g | 14.1 ± 0.4 fg | 14.7 ± 1.1 f | 19.7 ± 1.1 e | 24.5 ± 0.3 cd | 25.6 ± 0.2 c | 32.3 ± 0.2 b | 38.1 ± 1.3 a | 21.4 ± 0.6 de | 20.8 ± 0.8 de | 23.4 ± 1.1 ce | 22.3 ± 0.5 ce |
| FA | 125.2 ± 5.9 h | 141.6 ± 7.4 gh | 147.4 ± 7.7 fh | 162.4 ± 12.0 efg | 194.8 ± 1.1 cd | 206.7 ± 0.2 bc | 229.4 ± 1.8 b | 296.0 ± 1.1 a | 170.2 ± 5.0 defg | 163.3 ± 6.2 efg | 178.7 ± 1.3 ce | 173.0 ± 6.5 def |
| OC | 6.1 ± 0.1 f | 10.8 ± 0.3 e | 10.9 ± 0.0 e | 11.6 ± 0.2 de | 22.3 ± 0.5 c | 25.6 ± 0.2 b | 39.4 ± 0.9 a | 41.1 ± 1.1 a | 14.7 ± 1.1 d | 13.0 ± 0.6 de | 21.4 ± 0.6 c | 20.8 ± 0.8 c |
| EA | 3598.7 ± 9.69 i | 4687.2 ± 39.5 h | 6997.6 ± 121.5 g | 7692.1 ± 294.2 fg | 9262.1 ± 172.9 ce | 10,021.9 ± 331.6 c | 12,528.9 ± 105.7 b | 14,443.8 ± 202.6 a | 8461.4 ± 300.2 def | 8296.8 ± 253.4 ef | 9325.3 ± 12.8 cd | 9109.3 ± 144.2 ce |
| IS | 972.6 ± 14.3 f | 1313.3 ± 25.3 e | 1355.2 ± 16.0 de | 1464.2 ± 11.8 ce | 2099.4 ± 29.1 b | 2175.8 ± 170.1 b | 2407.5 ± 68.1 b | 2776.2 ± 16.0 a | 1658.9 ± 0.0 cd | 1526.14 ± 25.8 ce | 1760.7 ± 108.7 c | 1689.1 ± 3.0 c |
| RT | 587.7 ± 6.7 g | 600.5 ± 32.9 g | 781.3 ± 54.9 f | 809.1 ± 52.6 ef | 1161.6 ± 22.6 bc | 1189.2 ± 22.9 bc | 1332.4 ± 38.7 ab | 1449.0 ± 61.4 a | 961.0 ± 0.8 de | 854.5 ± 5.4 ef | 1103.9 ± 34.7 cd | 1036.5 ± 8.1 cd |
| RS | 7782.0 ± 129.0 i | 9776.1 ± 233.9 h | 11,368.9 ± 124.6 g | 11,776.2 ± 233.7 fg | 19,539.2 ± 300.3 c | 20,700.4 ± 363.7 b | 29,365.8 ± 394.6 a | 30,181.1 ± 167.3 a | 12,626.8 ± 116.8 f | 16,352.9 ± 112.6 e | 19,293.1 ± 176.9 c | 17,664.9 ± 19.2 d |
| HY | 1011.6 ± 76.0 h | 1443.2 ± 45.2 gh | 1910.8 ± 78.9 fg | 2056.6 ± 266.8 eg | 3927.3 ± 96.3 c | 4072.3 ± 108.6 c | 4988.2 ± 290.9 b | 5733.0 ± 166.9 a | 2685.3 ± 108.6 de | 2479.4 ± 99.1 def | 2944.75 ± 84.7 d | 2455.1 ± 86.3 def |
| NA | 885.0 ± 16.8 h | 1514.2 ± 19.9 g | 1628.2 ± 37.6 g | 2305.6 ± 16.8 f | 4608.0 ± 153.6 c | 4842.8 ± 40.2 c | 5405.2 ± 46.8 b | 6760.3 ± 47.6 a | 3618.2 ± 24.6 e | 2541.2 ± 23.9 f | 4055.9 ± 150.8 d | 3724.8 ± 10.2 de |
| QE | ND | ND | 1526.0 ± 51.0 h | 1626.4 ± 34.9 h | 7366.8 ± 53.0 c | 7535.8 ± 31.9 c | 9471.2 ± 85.9 b | 9912.0 ± 123.0 a | 2575.7 ± 15.9 f | 2067.0 ± 19.4 g | 5417.6 ± 39.7 d | 4609.8 ± 19.8 e |
| LU | ND | ND | ND | ND | 228.3 ± 2.1 c | 378.8 ± 0.4 b | 408.1 ± 0.3 a | 408.7 ± 14.1 a | 140.8 ± 3.3 de | 115.7 ± 3.4 e | 168.0 ± 4.3 d | 153.8 ± 10.2 d |
| NR | ND | ND | ND | ND | 80.5 ± 1.6 c | 89.1 ± 3.9 bc | 100.6 ± 1.7 ab | 102.1 ± 2.7 a | 36.2 ± 1.7 e | 29.9 ± 0.0 e | 61.8 ± 3.0 d | 52.8 ± 5.4 d |
| KA | ND | ND | ND | ND | 11,345.6 ± 62.0 d | 11,491.2 ± 12.3 c | 17,106.9 ± 56.9 b | 20,582.8 ± 19.6 a | ND | ND | ND | ND |
RF—reference compounds. LA—L. angustifolia hydroalcoholic extracts. ND—unidentified polyphenolic compound. TA—tannic acid; GA—gallic acid; PA—protocatechuic acid; CA—catechin; CF—caffeic acid; CH—chlorogenic acid; SY—syringic acid; EP—epicatechin; PC—p-coumaric acid; FA—ferulic acid; OC—o-coumaric acid; EA—ellagic acid; IS—isoquercetin; RT—rutin; RS—rosmarinic acid; HY—hyperoside; NA—naringin; QE—quercetin; LU—luteolin; NR—naringenin; KA—kaempferol. Values are presented as means ± SD, n = 3 per treatment group. Data without a common superscript letter differ (p < 0.05), as analyzed by one-way ANOVA and the TUKEY test.