Table 5.
Identification and quantification of polyphenolic compounds present in L. latifolia hydroalcoholic extracts.
RF | LL Hydroalcoholic Extracts (mg/kg Plant) | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
LL-1 | LL-2 | LL-3 | LL-4 | LL-5 | LL-6 | LL-7 | LL-8 | LL-9 | LL-10 | LL-11 | LL-12 | |
TA | 28.2 ± 0.9 e | 34.5 ± 1.6 de | 40.8 ± 2.8 ce | 43.9 ± 2.5 ce | 61.2 ± 2.9 bc | 69.6 ± 4.0 b | 95.4 ± 4.3 a | 97.3 ± 5.5 a | 47.1 ± 3.3 be | 45.5 ± 0.6 be | 59.2 ± 2.5 bcd | 50.3 ± 14.0 be |
GA | 7.0 ± 0.1 f | 7.3 ± 0.1 ef | 7.5 ± 0.2 ef | 8.8 ± 0.3 e | 12.3 ± 0.2 bd | 13.1 ± 0.4 bc | 13.7 ± 0.2 b | 16.5 ± 0.1 a | 11.7 ± 0.2 cd | 10.9 ± 0.6 d | 13.0 ± 0.7 bc | 12.4 ± 0.3 bd |
PA | 1234.1 ± 40.1 f | 1659.4 ± 5.0 e | 1945.6 ± 10.9 e | 3033.5 ± 32.1 d | 3945.2 ± 34.4 b | 4027.9 ± 87.5 b | 6879.5 ± 125.4 a | 6984.7 ± 125.9 a | 3562.3 ± 56.2 c | 3340.0 ± 59.5 cd | 3936.3 ± 32.3 b | 3573.4 ± 40.5 c |
CA | 103.5 ± 10.7 g | 151.8 ± 3.6 fg | 152.6 ± 20.8 fg | 164.2 ± 19.0 f | 263.8 ± 4.5 cd | 297.0 ± 2.4 ac | 320.7 ± 6.2 ab | 345.5 ± 1.8 a | 175.4 ± 0.5 ef | 166.8 ± 6.7 f | 278.3 ± 13.9 bc | 220.8 ± 8.7 de |
CF | 59.4 ± 4.6 f | 69.5 ± 0.4 f | 76.1 ± 3.8 ef | 88.4 ± 4.4 ef | 196.7 ± 1.4 b | 215.3 ± 27.1 ab | 229.8 ± 4.7 ab | 248.1 ± 5.0 a | 116.5 ± 0.0 ce | 95.0 ± 1.7 def | 139.5 ± 4.1 c | 134.6 ± 2.4 cd |
CH | 87.2 ± 3.2 f | 96.4 ± 0.5 ef | 103.0 ± 2.9 df | 103.0 ± 2.9 df | 123.3 ± 0.7 abc | 128.8 ± 5.0 abc | 130.9 ± 0.1 ab | 139.4 ± 1.4 a | 112.8 ± 5.1 cde | 103.3 ± 1.9 df | 120.0 ± 3.5 bd | 117.4 ± 6.7 bd |
SY | 302.4 ± 3.4 h | 526.6 ± 4.5 g | 893.4 ± 3.5 f | 1041.6 ± 23.6 ef | 1537.9 ± 23.7 c | 1562.4 ± 34.2 c | 1814.1 ± 25.9 b | 2023.9 ± 7.5 a | 1340.4 ± 64.9 d | 1120.4 ± 31.8 e | 1558.2 ± 42.3 c | 1449.0 ± 28.6 cd |
EP | ND | 60.7 ± 4.3 f | 119.6 ± 0.6 e | 156.3 ± 1.0 de | 261.3 ± 1.5 b | 281.8 ± 2.5 b | 295.8 ± 2.1 b | 395.1 ± 15.7 a | 168.1 ± 4.4 cd | 151.0 ± 1.4 de | 202.7 ± 19.9 c | 173.6 ± 11.5 cd |
PC | 39.7 ± 0.6 f | 52.1 ± 0.0 f | 57.1 ± 1.4 f | 65.1 ± 0.7 ef | 112.9 ± 4.7 cd | 127.3 ± 7.3 bc | 146.6 ± 7.4 ab | 156.5 ± 8.3 a | 92.1 ± 0.7 d | 90.5 ± 8.7 de | 104.2 ± 3.0 cd | 100.8 ± 3.5 d |
FA | 151.3 ± 6.8 g | 210.0 ± 24.1 fg | 239.1 ± 7.2 fg | 248.1 ± 2.0 fg | 489.3 ± 5.2 bd | 509.9 ± 43.8 bc | 568.8 ± 16.8 ab | 622.2 ± 46.7 a | 291.2 ± 4.0 ef | 272.4 ± 8.5 f | 410.4 ± 9.7 cd | 382.9 ± 3.4 de |
OC | 7.5 ± 0.2 e | 16.1 ± 1.4 de | 30.6 ± 1.2 cd | 44.9 ± 3.7 bc | 53.7 ± 3.3 b | 55.7 ± 2.6 b | 78.8 ± 8.2 a | 79.8 ± 1.2 a | 49.4 ± 1.3 b | 47.4 ± 3.0 b | 54.3 ± 0.1 b | 53.7 ± 1.0 b |
EA | 5642.8 ± 4.0 i | 7244.9 ± 121.6 h | 7655.6 ± 188.7 h | 9468.2 ± 19.6 g | 16,189.5 ± 181.4 cd | 17,389.9 ± 176.0 bc | 17,861.0 ± 154.2 b | 19,572.4 ± 480.3 a | 11,639.6 ± 282.5 f | 10,480.3 ± 294.9 fg | 15,547.2 ± 348.2 d | 13,113.9 ± 204.7 e |
IS | 811.7 ± 8.2 f | 996.5 ± 9.0 e | 1254.5 ± 70.5 d | 1322.3 ± 29.0 d | 1817.7 ± 76.0 ac | 1858.6 ± 28.6 ab | 1914.6 ± 13.6 a | 1950.0 ± 23.5 a | 1728.5 ± 12.9 bc | 1661.1 ± 9.0 c | 1949.9 ± 14.1 a | 1735.0 ± 14.3 bc |
RT | 162.2 ± 2.1 g | 179.2 ± 0.9 g | 265.8 ± 19.0 f | 307.1 ± 21.4 ef | 615.2 ± 0.3 c | 636.9 ± 0.4 c | 688.6 ± 6.6 b | 815.8 ± 13.8 a | 343.5 ± 11.0 e | 308.8 ± 13.4 ef | 484.2 ± 3.8 d | 464.1 ± 7.1 d |
RS | 10,733.0 ± 33.0 j | 16,950.2 ± 49.8 i | 21,590.4 ± 35.2 h | 21,797.8 ± 79.7 h | 30,411.3 ± 31.4 f | 34,181.0.8 ± 44.8 e | 36,134.7 ± 74.9 c | 39,841.0 ± 21.0 a | 34,159.8 ± 174.6 e | 24,298.9 ± 98.9 g | 38,892.1 ± 41.8 b | 35,692.0 ± 18.7 d |
HY | 632.3 ± 5.0 g | 1445.0 ± 6.0 f | 1851.4 ± 25.8 e | 2142.7 ± 88.3 de | 2632.2 ± 35.1 b | 2764.9 ± 48.6 b | 3235.1 ± 195.7 a | 3429.1 ± 109.1 a | 2492.6 ± 19.3 bd | 2191.1 ± 41.5 cde | 2564.3 ± 59.9 bc | 2512.2 ± 48.7 bd |
NA | 1606.0 ± 92.0 i | 1815.0 ± 48.8 i | 2633.2 ± 22.9 h | 3321.0 ± 54.9 g | 4878.7 ± 134.6 d | 6992.8 ± 39.9 c | 7621.0 ± 48.8 b | 9162.3 ± 28.2 a | 4421.3 ± 64.9 e | 4038.9 ± 94.6 f | 4977.9 ± 78.9 d | 4522.6 ± 22.7 e |
QE | ND | ND | 748.8 ± 2.48 f | 1212.7 ± 17.8 e | 3753.6 ± 164.8 c | 4365.6 ± 189.9 b | 4411.5 ± 15.9 ab | 4775.9 ± 7.9 a | 2771.2 ± 35.8 d | 2751.0 ± 22.8 d | 3629.2 ± 62.4 c | 2827.8 ± 7.2 d |
LU | 43.0 ± 0.4 g | 50.0 ± 1.9 g | 71.9 ± 2.2 f | 73.4 ± 1.9 f | 138.9 ± 1.3 b | 126.8 ± 2.9 bc | 188.9 ± 2.3 a | 203.5 ± 3.6 a | 96.5 ± 1.5 de | 86.5 ± 0.7 ef | 116.3 ± 9.9 cd | 101.2 ± 7.1 de |
NR | 50.5 ± 7.0 g | 74.2 ± 0.8 f | 87.1 ± 2.7 ef | 102.8 ± 2.5 de | 128.2 ± 3.4 bc | 134.4 ± 4.3 b | 143.0 ± 4.4 b | 164.4 ± 4.2 a | 109.2 ± 1.2 cd | 102.8 ± 3.3 de | 134.1 ± 6.5 b | 129.2 ± 2.4 bc |
KA | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND |
RF—reference compounds. LL—L. latifolia hydroalcoholic extracts. ND—unidentified polyphenolic compound. TA—tannic acid; GA—gallic acid; PA—protocatechuic acid; CA—catechin; CF—caffeic acid; CH—chlorogenic acid; SY—syringic acid; EP—epicatechin; PC—p-coumaric acid; FA—ferulic acid; OC—o-coumaric acid; EA—ellagic acid; IS—isoquercetin; RT—rutin; RS—rosmarinic acid; HY—hyperoside; NA—naringin; QE—quercetin; LU—luteolin; NR—naringenin; KA—kaempferol. Values are presented as means ± SD, n = 3 per treatment group. Data without a common superscript letter differ (p < 0.05), as analyzed by one-way ANOVA and the TUKEY test.