Table 3.
Reverse transcription quantitative PCR (RT-qPCR) Cq values from the positive urine and kidney samples across isolation attempts
| Samples | Cq value RT-qPCR | Cq value of the first cell passage | Cq value of the second cell passage | Cq value of the third cell passage | Cq value of the fourth cell passage | Cq value of the fifth cell passage |
|---|---|---|---|---|---|---|
| Us1 (cat Totò) | 29.78 | 23.48 | 24.22 | 32.42 | 39.02 | >40 |
| Us2 | 34.20 | >40 | NP | NP | NP | NP |
| Us3 | 35.01 | >40 | NP | NP | NP | NP |
| Us4 | 35.72 | >40 | NP | NP | NP | NP |
| Us5 | 39.53 | >40 | NP | NP | NP | NP |
| Us6 | 39.98 | >40 | NP | NP | NP | NP |
| Us7 | 33.66 | >40 | NP | NP | NP | NP |
| Us8 | 35.21 | >40 | NP | NP | NP | NP |
| Us9 | 36.00 | >40 | NP | NP | NP | NP |
| Us10 | 39.73 | >40 | NP | NP | NP | NP |
| Us11 | 37.78 | >40 | NP | NP | NP | NP |
| Pooled Us1 | 30.83 | >40 | NP | NP | NP | NP |
| Pooled Us2 | 29.66 | >40 | NP | NP | NP | NP |
| Ks1 | 33.86 | >40 | NP | NP | NP | NP |
| Ks2 | 37.53 | >40 | NP | NP | NP | NP |
| Ks3 | 38.21 | >40 | NP | NP | NP | NP |
| Ks4 | 32.80 | >40 | NP | NP | NP | NP |
Us = individual urine samples; pooled Us = pooled urine samples; Ks = kidney samples; NP = not performed