FIG. 2.

Alignment of RF2 amino acid sequences. The amino acid sequences of RF2 from B. subtilis (Bs) (this work and reference 35), S. coelicolor (Sc) (28), E. coli (Ec) (10), S. typhimurium (St) (21), and H. influenzae (Hi) (GenBank accession no. P43918) are shown. Amino acids that are identical in all five sequences are shown in bold type; the overall identity is 35%. The asterisks with numbers located above the B. subtilis sequences identify the amino acids changed in prfB1, -3, and -5. The frameshift region found in all of the sequences except that of S. coelicolor is marked with a dashed line above the B. subtilis amino acid sequence.