TABLE 2.

Sporulation of suppressed spoIIR mutants at different temperatures

Relevant genotypea	Spores per mlb		Relative increase in spores per ml
	37°C	42°C	37°C	42°C
spoIIR152	6.2 × 103	1.5 × 103
spoIIR152 prfB1	2.7 × 106	1.4 × 102	428	−11
spoIIR152 prfB2	2.8 × 106	1.2 × 106	444	800
spoIIR152 prfB3	9.8 × 105	1.1 × 105	156	73
spoIIR152 prfB4	1.8 × 106	4.0 × 105	290	267
spoIIR152 prfB5	4.3 × 105	1.8 × 105	69	120
spoIIR151	4.2 × 104	1.2 × 104
spoIIR151 prfB2	1.3 × 107	2.5 × 106	309	208
spoIIR74	4.0 × 105	1.3 × 105
spoIIR74 prfB3	5.9 ×107	1.3 × 107	148	100
spoIIR74 prfB4	1.4 × 108	2.6 × 107	350	200
spoIIR74 prfB5	3.0 × 107	1.7 × 107	75	131

^aStrains are isogenic sets derived from B. subtilis 168 strain BR151. 

^bSpores per milliliter were determined as heat-resistant spores per milliliter of culture. A strain with an insertion mutation in spoIIR produced less than 10 spores per ml.