TABLE 4.
Suppression of catA86 UGA-7 by prfB mutants
| prfBa mutation | CAT activityb
|
% of suppression | |
|---|---|---|---|
| C2 (WT)c | UGA-7d | ||
| prfB+ | 10.8 | 0.7 | 6 |
| prfB1 | 23.9 | 4.6 | 19 |
| prfB2 | 9.5 | 5.0 | 53 |
| prfB3 | 14.7 | 2.9 | 20 |
| prfB4 | 14.5 | 2.9 | 20 |
| prfB5 | 5.1 | 2.7 | 54 |
a
prfB+ is in strain BR151; prfB mutations are in derivatives of B. subtilis 168 strain BR151.
b
Specific activity of CAT is expressed as micromoles per minute per milligram of protein. Data are the average of four experiments, with specific activities varying ±10 to 15% between experiments. In each experiment, the relative level of expression between the different samples remained the same.
c
Strain containing pPL708C2 (see Materials and Methods).
d
Strain containing pPL708C2 UGA-7.