TABLE 2.
Cholesterol uptake in upc2-1 and upc2::URA3 strains
Strain | Relevant genotypea | Mean cholesterol uptake (μg of cholesterol/mg [dry weight] of cells) ± SD with plasmidb:
|
|
---|---|---|---|
YpH-1 | pUPC2 | ||
463-1D | UPC2 | 0.232 ± 0.041 | 0.214 ± 0.044 |
SC2-1C | upc2-1 | 4.68 ± 1.1 | 0.771 ± 0.059 |
CJ625 | upc2::URA3 | 0.160 ± 0.018 | 0.178 ± 0.024 |
CJ296 | UPC2/UPC2 | 0.174 ± 0.018 | 0.178 ± 0.024 |
CJ571 | upc2-1/UPC2 | 1.01 ± 0.065 | 0.226 ± 0.037 |
CJ573 | upc2-1/upc2::URA3 | 3.82 ± 0.27 | 0.601 ± 0.21 |
CJ577 | upc2-1/upc2-1 | 2.56 ± 0.79 | 0.573 ± 0.019 |
CJ581 | upc2::URA3/upc2::URA3 | 0.279 ± 0.022 | 0.470 ± 0.33 |
In this table ydr213::URA3 is designated upc2::URA3.
Plasmid YpH-1 is the control plasmid lacking the YDR213W open reading frame, and plasmid pUPC2 contains the entire YDR213W coding sequence with the native 5′ untranslated sequence.