Figure 5.
SCC-9 (HPV−) cells induced mobilization of intracellular NE in neutrophils. NE expression was evaluated through immunofluorescence using a secondary antibody conjugated with Alexa FluorTM 488 (green) and nuclear staining with DAPI (blue). Merged images are shown at 40× magnification (A), and the quantification is presented (B). Differences were found between C− and the other groups, except for C+. Three independent experiments were performed. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, **** p < 0.0001; one-way ANOVA, post hoc Games–Howell test. 16-E6: HaCaT keratinocytes transduced with HPV 16 E6, 16-E7: HaCaT keratinocytes transduced with HPV 16 E7, C−: negative control, C+: positive control, LPS: lipopolysaccharide, pLVX: HaCaT keratinocytes transduced with the empty pLVX-Puro vector, SCC-9: HPV− OSCC cell line, UPCI: HPV+ OSCC cell line.