TABLE 3.
LMW extracellular carbohydrate (expressed as percentage of total extracellular carbohydrate) generated by the addition of glycanase (final concentration of 200 ng/ml) to culturesa
| Strain genotype | % Extracellular carbohydrate in LMW form when indicated enzyme was added:
|
|||
|---|---|---|---|---|
| Throughout the course of culture incubation
|
Entirely upon inoculation of cultures
|
|||
| ExsH | ExoK | ExsH | ExoK | |
| exoK exsH | 54 | 9 | 87 | 41 |
| exoK exsH exoZ | 90 | 35 | 100 | 83 |
| exoK exsH exoH | 2 | 7 | 3 | 5 |
| exoK exsH exoH exoZ | 13 | 7 | 7 | 13 |
a
Under the incubation conditions used here, approximately 97% of total extracellular carbohydrate is succinoglycan.