Table 2.
Top 20 significant biological clusters enriched in the DCD-associated gene co-expression network.
| Biological Cluster | Parental Biological Term | p-Value (Log10) |
|---|---|---|
| Modulation of chemical synaptic transmission (GO:0050804) |
Synaptic signaling | −26.82 |
| Synaptic signaling (GO:0099536) | Synaptic signaling | −21.91 |
| Cell junction organization (GO:0034330) | Cellular process—Cellular component organization | −15.87 |
| Neuronal system (R-HSA-112316) | Synaptic signaling—Chemical synaptic transmission | −14.69 |
| Behavior (GO:0007610) | Multicellular organismal process | −13.99 |
| Regulation of cell projection organization (GO:0031344) |
Cellular process—Cellular component organization | −13.61 |
| Neuron projection development (GO:0031175) | Nervous system development | −12.77 |
| Regulation of ion transport (GO:0043269) | Cellular process—Transport | −9.48 |
| Protein localization to synapse (GO:0035418) | Cell process—Cellular localization | −8.94 |
| L1CAM interactions (R-HSA-373760) | Nervous system development—Axon guidance | −8.89 |
| Metal ion transport (GO:0030001) | Cellular process—Transport | −7.57 |
| Actin filament-based process (GO:0030029) | Cellular process | −7.00 |
| Cell-cell adhesion (GO:0098609) | Cellular process—Cell adhesion | −6.35 |
| Action potential (GO:0001508) | Biological regulation—Regulation of biological quality | −6.30 |
| Neuromuscular process (GO:0050905) | Nervous system process | −5.94 |
| Brain development (GO:0007420) | Nervous system development | −5.42 |
| Protein localization to membrane (GO:0072657) |
Cellular process—Cellular localization | −5.32 |
| Calcium-ion regulated exocytosis (GO:0017156) | Cellular process—Export from cell | −5.32 |
| Regulation of glutamatergic synaptic transmission (GO:0051966) | Synaptic signaling | −4.91 |
| Locomotory behavior (GO:0007626) | Multicellular organismal process—Behavior | −4.78 |
Top 20 most significantly enriched clusters of biological pathways for the 12 DCD-associated genes. In Metascape, biological pathways with similar biological functions are clustered together. The biological pathway with the highest p-value becomes the cluster representative, after which the cluster is named. A unique biological pathway identifier is given in brackets for each pathway (i.e., GO:.. and R-HSA-..); more information can be found online: https://geneontology.org/docs/GO-term-elements and https://reactome.org/ (both accessed on 30 May 2022). The p-value for each cluster is expressed in log10, corrected for multiple testing. Parental biological terms are given for each cluster, indicating the broader terms of which the specific biological pathway (i.e., cluster representative) is part, i.e., “cell junction organization” is a form of “cellular component organization”, which in turn is a “cellular process”. This information can be found online: https://geneontology.org/docs/GO-term-elements and https://www.informatics.jax.org/vocab/gene_ontology (both accessed on 30 May 2022). Based on the parental biological terms, we identified three main biological themes: (1) cellular processes, (2) neural signaling, and (3) nervous system development. The complete list of enriched clusters and biological pathways is shown in Supplementary File S6.