FIG. 2.

Plasmid transactivation profiles of MC4100 harboring λ lysogens and transactivation plasmids bearing the S105 gene with inserts encoding an oligohistidine tag at either the N or C terminus. (A) Inductions of MC4100 [λ Δ(SR)] cells harboring pKB1 (Sam7) (○), pS105 (•), pτS105 (□), and pS105τ (⧫) were performed as described in Materials and Methods. Similar plasmid transactivations were performed with the following different lysogens: MC4100 [λ 111] (Sam7) (B), MC4100 [λ S⁺] (C), and MC4100 [λ S105] (D).