FIG. 2.

Levels of yklA- and ykzA-specific mRNA before and after the imposition of different stresses. RNA was prepared from the wild-type strain 168 at the times indicated on the x axes. Specific RNAs were detected with digoxigenin-labeled RNA probes, and the intensities of the signals were quantified with a laser densitometer as described previously (36). The amount of RNA present during exponential growth was set to one. The induction ratios of yklA (solid bars) and ykzA (shaded bars) at the different time points are displayed. All stresses were applied at time zero with the exception of glucose limitation, where zero indicates the point at which the culture ceased to grow.