FIG. 3.

Northern blot analysis of ykzA. Equal amounts of total RNA (10 μg) prepared from the wild-type 168 or the isogenic sigB mutant (ML6) before (co) and at different times (in minutes) after exposure to stress were separated on denaturing gels, transferred onto a positively charged nylon membrane, and hybridized with digoxigenin-labeled RNA probe specific for ykzA.