Figure 4.

Smad4^KO BRAF^V600E/+ tumor organoids have invasive behavior in 2D culture. (A) Viability of organoids plated without BME (*** = adj p-value = 0.002; **** = adj p-value < 0.0001, ANOVA). (B) Smad4^KO BRAF^V600E/+ adjacent normal and tumor organoids are capable of surviving on plastic and formed a 2D monolayer. Scale bar = 1 mm. (C) Diagram of transwell assay for invasive potential. (D) Smad4^KO BRAF^V600E/+ RosaGFP tumor organoids were capable of migrating through transwell after 3 weeks and forming 2D colonies (Arrows). Images representative of 3 biological replicates. Scale bar = 0.5 mm.