Skip to main content
. 2023 Dec 13;15(24):5820. doi: 10.3390/cancers15245820

Figure 6.

Figure 6

SMAD4 suppresses cell cycle and proliferation in 2D culture. (A) Heatmap of Dox-treated orgnaoids in 2D monolayer. (B,C) DAVID analysis of genes upregulated (B) and downregulated (C) in SMAD4-expressing tumor organoids grown in the absence of BME R1. (D) EdU stain of tumor organoids in 2D cultures, images representative of 3 biological replicates. Scale bar = 1 mm. (E) Quantificaiton of EdU-positive cells in organoids (p-value reported, Student’s t-test). (F) GSEA analysis reveals cell cycle is downregulated upon expression of SMAD4.