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Significantly different MGMT promoter/enhancer methylation levels between younger (<60 years) and older (≥60 years) patients. MGMT (a) promoter, (b) enhancer 1, (c) enhancer 2, (d) enhancer 3, and (e) enhancer 4 regions. prom M—promoter methylated (purple), UM—unmethylated (green), UM + M (blue) samples. Significance levels: * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. Data points represent the mean of two independent PCR-PSQ runs.
