FIG. 5.
Structure of the comF-flgM operon and construction of a comF-flgM::pJL010 insertion mutant. (A) flgM is located within an operon containing the promoter PD-1, immediately downstream of the comF operon. comF is a B. subtilis late competence operon. Transcription of comF is driven by a single ςA-type promoter, utilization of which is dependent on ComK. The flgM operon contains orf139, flgM, orf160, and flgK. (B) The mutant was constructed by insertion of a plasmid (pJL010) carrying comFC and orf139 fragments by a single-recombination mechanism into the region containing the junction between the comF and flgM operons.