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. Author manuscript; available in PMC: 2023 Dec 22.
Published in final edited form as: Cell Rep. 2023 Oct 19;42(10):113302. doi: 10.1016/j.celrep.2023.113302

Figure 2. FAP is required for TWIST1-induced breast cancer metastasis.

Figure 2.

(A) qRT-PCR measurement of FAP mRNA levels in HMLER-TWIST1 cells expressing shRNAs targeting FAP.

(B) Western blotting analysis on lysates obtained from HMLER-TWIST1 cells as indicated.

(C) Nude mice were injected subcutaneously with GFP + HMLER-TWIST1 cells expressing a control vector (shCtrl) or shRNAs targeting FAP. Tumor diameters were measured as indicated. Once the tumors reached 2 cm in diameter, they were harvested and weighed as shown in (D).

(E) Quantification of GFP-positive cells in each individual lung. N = 10 mice per group. **p < 0.01, Student’s t-test. Error bars in (A, C, D and E) represent the SD.

(E) Representative images of lungs of nude mice injected with HMLER-TWIST1 cells expressing the indicated shRNAs. Scale bar, 1 mm. See also Figure S1.