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. 1998 Aug;180(16):4258–4269. doi: 10.1128/jb.180.16.4258-4269.1998

FIG. 5.

FIG. 5

Western immunoblot analysis of R. capsulatus wild-type and CbbR-minus strains. Purified Synechococcus sp. strain PCC 6301 RubisCO and purified R. sphaeroides form II RubisCO were loaded into lanes 1 and 11, respectively. Crude extracts were loaded (approximately 10 μg of protein) as follows: lane 2, photoheterotrophically grown SB1003; lane 3, photoautotrophically grown SB1003; lane 4, photoheterotrophically grown SBRI−; lane 5, photoautotrophically grown SBRI−; lane 6, photoheterotrophically grown SBRI− with pVK::CbbRI; lane 7, photoautotrophically grown SBRI− with pVK::CbbRI; lane 8, photoheterotrophically grown SBRII−; lane 9, photoheterotrophically grown SBRII− with plasmid pVK::CbbRII; lane 10, photoautotrophically grown SBRII− with plasmid pVK::CbbRII. Blots were incubated with antibody raised against Synechococcus strain PCC 6301 RubisCO (A) and R. sphaeroides form II RubisCO (B). The figure was generated as described in the legend to Fig. 3.