TABLE 2.
Growth rates, RubisCO activities, and PRK activities of R. capsulatus wild-type and cbb mutant and complemented strains
| Strain | Growth conditiona | Doubling timeb (h) | Activity (nmol min−1
mg−1)
|
|
|---|---|---|---|---|
| RubisCO | PRK | |||
| SB1003 (wild type) | MAL | 5.0 | 30.7 ± 16.5 | 112.9 ± 12.4 |
| PA | 12.5 | 459.6 ± 51.0 | 451.1 ± 9.8 | |
| MAL/DMSO | 12.5 | 32.4 ± 8.4 | 95.3 ± 14.2 | |
| SBI− (cbbL) | MAL | 6.5 | 51.3 ± 3.1 | 76.5 ± 15.7 |
| PA | 15.5 | 362.8 ± 5.6 | 489.9 ± 36.2 | |
| SBII− (cbbM) | MAL | 7.0 | 31.7 ± 1.3 | 193.0 ± 25.3 |
| PA | 15.5 | 313.6 ± 18.4 | 506.3 ± 28.9 | |
| SBI-II (cbbL− cbbM) | MAL/DMSO | 14.5 | 0.0 | 25.7 ± 5.8 |
| SBI-II(pRPSFI-I) | MAL | 9.5 | 34.5 ± 0.4 | NDc |
| PA | 49.5 | 269.4 ± 13.9 | ND | |
| SBI-II(pRPSFII-I) | MAL | 10.5 | 29.8 ± 4.9 | ND |
| PA | 40.0 | 492.8 ± 39.2 | ND | |
| SBP− (cbbP) | MAL/DMSO | 8.0 | 3.0 ± 1.1 | 0.0 |
| SBP−(pRPS::RsPIA) | MAL | 16.5 | 12.8 ± 1.8 | 922.8 ± 202.4 |
| SBRI− (cbbRI) | MAL | 3.3 | 19.2 ± 0.6 | ND |
| PA | 13.3 | 237.8 ± 33.7 | ND | |
| SBRI−(pVK::CbbRI) | MAL | 2.9 | 16.2 ± 0.7 | ND |
| PA | 12.6 | 256.4 ± 21.5 | ND | |
| SBRII− (cbbRII) | MAL | 10.5 | 10.1 ± 1.9 | 11.3 ± 1.3 |
| SBRII−(pVK::CbbRII) | MAL | 7.5 | 11.8 ± 0.1 | 17.8 ± 3.4 |
| PA | 47.0 | 49.1 ± 3.5 | 64.5 ± 6.9 | |
a
MAL, photoheterotrophic growth on malate; PA, photoautotrophic growth on 1.5% CO2–98.5% H2; MAL/DMSO, photoheterotrophic growth on malate in the presence of 30 mM DMSO.
b
Average of at least two independent determinations, with no more than a 15% discrepancy for any one growth rate.
c
ND, not determined.