TABLE 1.
Assessing the role of the ADC pathway in arginine catabolism in E. coli
| Medium | OD600 at saturation | Arg depleted (mM)a | Urea accumulated (mM) | % Arg to urea |
|---|---|---|---|---|
| Minimalb | ||||
| Arginine | 2.66 | 5.0 | 0.15 | 3 |
| NH4+-arginine | 4.74 | 1.1 | 0.40 | 36 |
| NH4+ | 4.74 | NAc | 0.13 | NA |
| Complex acidicd | ||||
| Aerobic growth | 4.12 | NAd | 0.12 | NA |
| Anaerobic growth | 0.95 | NAd | 0.21 | NA |
a
Calculated as starting concentration (0.2% = 11.5 mM) minus the concentration at the indicated optical density.
b
Cells were grown in minimal medium with 0.4% glucose and 0.2% of each indicated nitrogen source.
c
NA, not applicable.
d
Cells were grown in buffered modified Falkow arginine medium, pH 5.5 (32). This medium contains peptides. The arginine from these peptides might be used before the added arginine. Since the urea produced may result from degradation of the arginine in the peptides, it was not considered possible to compare the urea produced to the arginine consumed.