TABLE 4.
Enzyme activities in wild-type E. coli grown with different carbon sources
| Mediuma | Enzyme activity, μmol/h/mg of protein (n)b, of:
|
|
|---|---|---|
| Dihydrolase | Desuccinylase | |
| Aspartate | 0.33 ± 0.07 (2) | 0.69 ± 0.16 (3) |
| Aspartate-NH4+ | 0.28 ± 0.026 (2) | 0.68 ± 0.15 (3) |
| Succinate-NH4+ | 0.10 ± 0.009 (2) | 0.254 ± 0.008 (3) |
| Glycerol-NH4+ | 0.07 ± 0.010 (2) | 0.090 ± 0.003 (2) |
| Glucose-NH4+ | 0.03 ± 0.03 (2) | 0.01 ± 0.01 (2) |
a
Cells were grown in minimal medium with 1% aspartate, 0.4% of the other carbon sources, and 0.2% ammonia.
b
See footnote b of Table 2.