Skip to main content
. 2023 Dec 6;24(24):17186. doi: 10.3390/ijms242417186

Figure 6.

Figure 6

Recovery of bioenergetic in MELAS neurons. (A) Intracellular ATP content was determined in each group after oligomycin treatment (n = 12). (B) Intracellular lactate levels were determined in each group after oligomycin treatment (n = 12). (C) Seahorse XFp metabolic flux analysis of mitochondrial ATP production rates and glycolytic ATP production rates in each group of neurons (n = 8). (D) XFp ATP rate index calculated from data in panel C (n = 8). (E) Representative images of neurons from each group. Neurons in the co-culture group were co-cultured with REC/MSCs for 24 h; neurons from each group were collected and seeded into 96-well plates, cultured for 1 week, and then tested. Scale bars, 100 μm. (F) The CCK-8 assay was used to detect viability in each group (n = 9). Data represent the mean ± standard deviation (SD) of three independent experiments. ns, not significant. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.