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. 2005 Apr 1;19(7):788–793. doi: 10.1101/gad.332805

Figure 3.

Figure 3.

The HD trafficking signal is conserved in closely related class I KNOX homeodomain proteins, and promotes KN1 mRNA movement. (A) Phylogenetic tree of homeodomain sequences: LeT6 (accession no. T04317), BLR (AAP93641), BEL1 (AAM62510), STM (Q38874), KNAT1 (AAM03027), KNAT2 (P46640), KNAT3 (P48000), KNAT4 (P48001), KNAT5 (P48002), KNAT6 (NP_850951), KNAT7 (NP_564805). (B). Trichome rescue assays were performed using HD regions corresponding to KN1256–359. Descriptions of the chart are as in Figure 2C. (C) RNA trafficking assay. Stop codons are shown as a red asterisk after the GL1 coding region. The shorter KN1 regions shown in constructs in lines 2, 4, and 7 correspond to KN1256–359. The number of T1 plants showing trichome rescue/total number of T1 plants observed (rescue rate, percent) is shown on the right side of each construct. (3′)3′ untranslated region (UTR) of KN1 or of nopaline synthase (see Materials and Methods).