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. 2023 Dec 6;24(24):17206. doi: 10.3390/ijms242417206

Figure 3.

Figure 3

Figure 3

Figure 3

Effects of EVs on the metastasis-related properties in cultured cell lines of J774.1 and CTLL-2. (A) Flow cytograms of J774.1 cells loaded with EVs labeled with SYTOTMRNASelectTM (Ex: 490 nm, Em: 530 nm) detected by an FITC detector. a: J774.1 cells containing no EVs, b: Nanog+F10 EV-loaded cells, c: iPS EV-loaded cells. The P1 fraction was sorted for the invasion test. (B) Number of Nanog+F10 cells that invaded Matrigel and passed through the Transwell® membrane. mean ± SD, n = 3, *** p < 0.001. (C) Flow cytograms of J774.1 cells containing PKH26-labeled EVs and/or YG-beads. PKH26 (Ex: 551 nm, Em: 567 nm) and YG-beads (Ex: 441 nm, Em: 486 nm) were detected using PE and FITC detectors, respectively. (D) The number of J774.1 cells that phagocytosed YG-beads. mean ± SD, n = 3, * p < 0.05. (E) Flow cytograms of CTLL-2 cells loaded with EVs labeled with SYTOTMRNASelectTM. EV-loaded cells mainly existed in the P1 fraction and were sorted for the cytotoxicity test. (F) The effects of EVs on the cytotoxicity of CTLL-2 cells against Nanog+F10 cells. mean ± SD, n = 3, *** p < 0.001.