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. 2005 Apr 1;19(7):827–839. doi: 10.1101/gad.1286005

Figure 7.

Figure 7.

Regulation of HDAC3 phosphorylation by PP4. (A) HeLa cells were transfected with plasmids encoding Flag-HDACs and PP4 as indicated. Extracts were then immunoprecipitated (IP) with anti-Flag antibodies and immunoblotted (IB) with anti-phosphoserine antibodies (top panels) or with anti-Flag antibodies (bottom panels). (B) HeLa cells expressing either Flag-HDAC3 or Flag-HDAC2 were treated with (+) or without (–) 50 nM fostriecin. Cell extracts were immunoprecipitated and immunoblotted as in A.