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. 2023 Dec 12;24(24):17390. doi: 10.3390/ijms242417390

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© 2023 by the author.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A) Structure of LPS of E. coli 4s strain (O22 type with additional glucosylation) and its mutants lacking glucosylation (GTR-mutant) or O-unit O acetylation (wclK-). Dashed lines: the LPS structure in rough mutants (as exemplified by wclH-) and deep-rough mutants lacking outer (waaG-) or most of the inner core (waaC-). (B) LPS gel profile of the strains indicated in panel A: the wild-type strain (lane 1), non-acetylated OPS wclK- mutant (lane 2), non-glucosylated GTR- mutant (lane 3), rough wclH- (lane 4) and two deep-rough mutants waaG and waaC (lanes 5 and 6). Modified from [12], Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/ accessed on 6 November 2023).