Table 2.
Comparison between observed and expected percentages of the VOCs for Twist mixtures and wastewater samples.
MiSeq | MinION | ||||||||
---|---|---|---|---|---|---|---|---|---|
R9.4.1 | R10.4.1 Singleplex | R10.4.1 Duplex | |||||||
LoFreq | Freyja | LoFreq | Freyja | LoFreq | Freyja | LoFreq | Freyja | ||
Twist mixtures | Spearman rank correlation | 0.962 | 0.978 | 0.938 | 0.978 | 0.960 | 0.969 | 0.912 | 0.812 |
p-value | p < 0.05 | p < 0.05 | p < 0.05 | p < 0.05 | p < 0.05 | p < 0.05 | p < 0.05 | p < 0.05 | |
Mean deviation | 6.6 ± 5.9% | 5.8 ± 5.2% | 7.1 ± 7.1% | 5.6 ± 4.7% | 6.7 ± 5.4% | 6.8 ± 5.6% | 10.0 ± 9.0% | 10.0 ± 9.0% | |
Wastewater samples | Spearman rank correlation | 0.747 | 0.739 | 0.765 | 0.813 | 0.752 | 0.818 | 0.834 | 0.790 |
p-value | p < 0.05 | p < 0.05 | p < 0.05 | p < 0.05 | p < 0.05 | p < 0.05 | p < 0.05 | p < 0.05 | |
Mean deviation | 20.3 ± 13% | 21.3 ± 16% | 21.3 ± 14% | 17.9 ± 16% | 20.2 ± 15% | 16.9 ± 16% | 23.0 ± 19% | 24.0 ± 19% |