Figure 6.

Activating-receptor-expression phenotyping. On the day of harvest, 6 h after electroporation, all effector cells were stained with a mix of fluorophore-conjugated mAbs directed to stain a panel of selected NK-expressed activating receptors, including APC-conjugated αNKp30 mAb, FITC-conjugated αNKp44 mAb, PE-Vio770-conjugated αNKp46 mAb, APC-Vio770-conjugated αNKG2D mAb, and PE-conjugated αCD62. Stainings were followed by the addition of the Helix viability dye immediately prior to analysis by flow cytometry.