TABLE 3.
Intra-assay accuracy of NK-1R real-time RT-PCR for the specimensa
| Replicate | NK-1R mRNA (copy no.)
|
|||||||
|---|---|---|---|---|---|---|---|---|
| D1
|
D2
|
D3
|
Cell line
|
|||||
| PBL | MDM | PBL | MDM | PBL | MDM | IM9 | NT-2N | |
| 1 | 3,090 | 1,865 | 9,362 | 1,846 | 20,136 | 3,241 | 171,472 | 1,717,887 |
| 2 | 2,421 | 1,640 | 8,448 | 1,522 | 16,607 | 3,139 | 106,608 | 1,435,145 |
| 3 | 3,012 | 1,361 | 9,856 | 1,858 | 18,763 | 3,478 | 110,087 | 1,363,270 |
| 4 | 2,843 | 1,424 | 9,606 | 1,419 | 16,607 | 3,733 | 120,444 | 1,425,958 |
| Mean (± SD) | 2,842 (± 299) | 1,573 (± 229) | 9,318 (± 614) | 1,661 (± 224) | 18,022 (± 1,730) | 3,398 (± 265) | 127,153 (± 3,0125) | 1,485,565 (± 158,40) |
| CVb (%) | 10.52 | 14.55 | 6.58 | 13.48 | 9.60 | 7.80 | 23.69 | 1.10 |
a
D, donor; healthy peripheral blood. Abbreviations: PBL, peripheral blood lymphocytes; MDM, monocyte-derived macrophages; IM9, human B lymphoblasts; NT-2N, human neuronal cells derived from Ntera2/cl.D1 (NT2) cells.
b
CV, coefficient of variation.