TABLE 1.

Production of standard sera in BALB/cj mice

Pathogen	n	Inoculation route-dosea			Seroconversionb (% positive)
		Primary	Secondary	Final	ELISA	Multiplex
Reo-3	24	i.n.−1.0 TCID50	i.n.−1.0 TCID50	None	100	100
Sendal virus	4	i.n.−1 μg/10 μl	None	None	100	100
	18	i.n.−1 μg/10 μl	i.n.−1 μg/10 μl	None	100	100
GD7	3	i.n.−2 × 106 PFU/10 μl	None	None	100	100
	4	i.n.−6 × 106 PFU/10 μl	None	None	100	100
	4	i.n.−3 × 106 PFU/10 μl	i.n.−6 × 106 PFU/10 μl	None	100	100
	4	i.n.−6 × 106 PFU/10 μl	i.n.−6 × 106 PFU/10 μl	None	100	100
	4	i.n.−2 × 106 PFU/10 μl	i.n.−2 × 106 PFU/10 μl	i.n.−6 × 106 PFU/10 μl	100	100
MHV	10	d.b.	None	None	60	50
	12	d.b.	d.b.	None	100	100
	4	5 d.b. inoculations at 2-wk intervals			100	100
	15	6 d.b. inoculations at 2-wk intervals			100	100
EDIM	11	g.l.−1 μg/100 μl	i.p.−1 μg/100 μl	i.p.−1 μg/100 μl	100	100
	4	g.l.−3 μg/100 μl	g.l.−3 μg/100 μl	i.p.−3 μg/100 μl	100	100
	4	g.l.−6 μg/100 μl	g.l.−6 μg/100 μl	i.p.−6 μg/100 μl	100	100
	3	g.l.−9 μg/100 μl	g.l.−9 μg/100 μl	i.p.−9 μg/100 μl	100	100
M. pulmonis	4	i.n.−40 μg/10 μl	i.n.−40 μg/10 μl	i.p.−40 μg/10 μl	100	100
	4	i.n.−77 μg/10 μl	i.n.−77 μg/10 μl	i.p.−77 μg/10 μl	100	100
	4	i.n.−150 μg/10 μl	i.n.−150 μg/10 μl	i.p.−150 μg/10 μl	100	100
	12	i.p.−150 μg/10 μl	i.p.−150 μg/10 μl	None	92	100
Vaccinia virus	24	i.p.−1 μg/100 μl	i.p.−1 μg/100 μl	i.p.−1 μg/100 μl	91	91
MMV	12	i.n.−1.0 TCID50	i.n.−2.0 TCID50	i.p.−2.0 TCID50	41	41
	12	i.n.−1.0 TCID50	i.p.−2.0 TCID50	i.p.−2.0 TCID50	75	75
	24	i.p.−3.0 TCID50	i.p.−3.0 TCID50	i.p.−3.0 TCID50	100	100

^ai.n., intranasal; d.b., dirty bedding; g.l., gastric lavage; TCID₅₀, 50% tissue culture infectious dose.

^bFor simplicity, only the final seroconversion results are presented.