TABLE 2.

Production of standard sera in C57BL/6j mice

Pathogen	n	Inoculation route-dosea			Seroconversionb (% positive)
		Primary	Secondary	Final	ELISA	Multiplex
Reo-3	23	i.n.−1.0 TCID50	i.n.−1.0 TCID50	None	100	100
Sendai	23	i.n.−1 μg/10 μl	None	None	100	100
GD7	2	i.n.−2 × 106 PFU/10 μl	None	None	100	100
	20	i.n.−6 × 106 PFU/10 μl	None	None	73	77
	2	i.n.−2 × 106 PFU/10 μl	i.n.−2 × 106 PFU/10 μl	i.n.−6 × 106 PFU/10 μl	0	0
MHV	28	d.b.	None	None	46	46
	4	4 d.b. inoculations at 2-wk intervals			100	100
	20	6 d.b. inoculations at 2-wk intervals			100	100
EDIM	12	g.l.−1 μg/100 μl	i.p.−1 μg/100 μl	i.p.−1 μg/100 μl	100	100
	12	i.p.−1 μg/100 μl	i.p.−1 μg/100 μl	None	100	100
M. pulmonis	4	i.n.−40 μg/10 μl	i.n.−40 μg/10 μl	i.p.−40 μg/10 μl	100	100
	4	i.n.−77 μg/10 μl	i.n.−77 μg/10 μl	i.p.−77 μg/10 μl	100	100
	4	i.n.−150 μg/10 μl	i.n.−150 μg/10 μl	i.p.−150 μg/10 μl	100	100
	12	i.p.−150 μg/10 μl	i.p.−150 μg/10 μl	None	92	100
Vaccinia virus	21	i.p.−1 μg/100 μl	i.p.−1 μg/100 μl	i.p.−1 μg/100 μl	90	100
MMV	12	i.n.−1.0 TCID50	i.p.−1.0 TCID50	i.p.−1.0 TCID50	100	100
	12	i.n.−1.0 TCID50	i.n.−2.0 TCID50	i.p.−1.0 TCID50	83	91

^ai.n., intranasal; d.b., dirty bedding; g.l., gastric lavage; TCID₅₀, 50% tissue culture infectious dose.

^bFor simplicity, only the final seroconversion results are presented.