TABLE 4.
Multiplex microbead assay on mixed, singly positive mouse sera from a
| Serum sample | Dilutionb | MFI ratio of antigen beads to BSA beads for antigen-coated microbead set:
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Vaccinia virus | NCDV | Reo-3 | MP | MHV | Sendai virus | GD7 | MPV | CMV | MMV | ||
| Background | 0.28 | 0.23 | 0.31 | 0.13 | 0.26 | 0.58 | 0.24 | 0.17 | 0.25 | 0.50 | |
| All sera | 1:250 | 41.8 | 25.3 | 97.6 | 10.9 | 7.40 | 70.3 | 5.65 | 4.45 | 13.1 | 3.48 |
| Ectro (−) | 1:250 | 0.33 | 23.1 | 93.0 | 12.5 | 6.00 | 60.8 | 5.60 | 4.30 | 12.8 | 3.30 |
| EDIM (−) | 1:250 | 42.1 | 0.39 | 93.9 | 12.0 | 6.70 | 65.23 | 5.60 | 4.00 | 13.2 | 3.10 |
| Reo-3 (−) | 1:250 | 43.1 | 23.92 | 0.29 | 12.8 | 7.34 | 69.6 | 6.06 | 4.62 | 13.4 | 3.37 |
| MP (−) | 1:250 | 55.3 | 32.7 | 135 | 0.24 | 9.70 | 89.0 | 8.11 | 6.00 | 17.1 | 3.50 |
| MHV (−) | 1:250 | 41.0 | 23.7 | 96.8 | 12.1 | 0.82 | 65.9 | 5.67 | 4.21 | 12.6 | 3.52 |
| Sendai virus (−) | 1:250 | 45.8 | 24.2 | 100 | 12.7 | 7.14 | 0.65 | 6.20 | 4.60 | 13.0 | 3.10 |
| GD7 (−) | 1:250 | 43.6 | 24.1 | 96.0 | 12.0 | 6.80 | 66.4 | 0.04 | 4.30 | 12.8 | 3.27 |
| MPV (−) | 1:250 | 43.5 | 22.8 | 96.5 | 11.6 | 6.81 | 62.4 | 5.64 | 0.00 | 12.7 | 3.20 |
| CMV (−) | 1:250 | 45.6 | 23.8 | 93.7 | 10.8 | 6.11 | 68.0 | 5.50 | 4.22 | 0.48 | 2.90 |
| MMV (−) | 1:250 | 41.0 | 22.2 | 100 | 11.8 | 7.01 | 66.7 | 7.70 | 4.13 | 12.4 | 1.56 |
a
Results are averages of two independent experiments. MP, M. pulmonis; Ectro, ectromelia virus. Boldface indicates the least specific results.
b
Final dilution of each serum sample in the mixture.