TABLE 1.
Plasmids used in this study
Plasmid | Descriptiona | Reference |
---|---|---|
pSK41 | 46.4-kb conjugative plasmid; Nmr EbQar Gmr Tmr Kmr Tra+ | 11 |
pJE1 | 50.0-kb conjugative plasmid; Nmr EbQar Gmr Tmr Kmr Tpr Tra+ | 9, 11 |
pUW3626 | 54.4-kb conjugative plasmid; Nmr EbQar Gmr Tmr Kmr Pcr Tra+ | 3 |
pSK5093 | pSK41 deletion mutant lacking region between IS25741B and IS25741E; Nms EbQas Gmr Tmr Kmr Tra− | This study |
pSK5094 | pSK41 deletion mutant lacking region between IS25741E and IS25741G; Nmr EbQar Gms Tms Kms Tra+ | This study |
Abbreviations: EbQar, resistance to ethidium bromide and quaternary ammonium compounds; Gmr Tmr Kmr, resistance to gentamicin, tobramycin, and kanamycin; Nmr, neomycin resistance; Pcr, penicillin resistance; Tpr, trimethoprim resistance; Tra, conjugative transfer. s = sensitive.