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Micelle safety in vitro. HeLa and SH-SY5Y cells were challenged for (A) 24 h and (B) 48 h with increasing concentrations of blank DSPE-PEG2000 micelles and analysed using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide) assay. Cells were seeded in a 96-well plate at concentrations of 3000 or 6000 cells/well, respectively, and were maintained in culture medium (100 µL) for 24 h before the treatment.
