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. 2023 Dec 14;24(24):17478. doi: 10.3390/ijms242417478

Figure 6.

Figure 6

Micelles extend the activity of RAD001 in vitro. Ev-sol and Ev-mic activity on (A) HeLa and (B) SH-SY5Y cells was measured using the MTT assay. HeLa and SH-SY5Y cells were seeded into a 96-well plate at concentrations of 3000 cells/well and 6000 cells/well, respectively, and were maintained in culture medium (100 µL) for 24 h before the treatment. Ev-sol and Ev-mic were used fresh and after incubation at 37 °C, 25 °C, and 4 °C for 7, 14, 35, 50, and 77 days. Untreated, DMSO-treated (Veh), and blank micelle-treated (Empty mic) cells were employed as negative controls, while fresh RAD001 solution-treated cells (Ev-sol fresh) were used as positive controls. All treatments were performed at the same equivalent concentration of RAD001 (5 nM). Data are representative of three independent experiments. * p < 0.001, ** p < 0.01.