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. 2023 Dec 11;24(24):17354. doi: 10.3390/ijms242417354

Figure 2.

Figure 2

s17 inhibits HIV replication at the post-integration DNA repair step. (A) HIV vector washout experiment demonstrating no influence of s17 on the vector entry. s17 was added to 293T cells at indicated concentrations simultaneously with the HIV_wt vector or after vector washing out. At 24 h post-transduction, the luciferase expression level was measured. Data were normalized to DMSO-treated samples independently for both types of experiments. (B–E) The effect of s17, Nu7441 and Ku-55933 on the cell transduction efficiency with HIV_wt (B), total (C) or integrated (D) HIV cDNA level and post-integration DNA repair efficiency (E). (F) Transduction efficiency of 293T cells with HIV_wt or HIV_mut carrying IN_E212A/L213A variant unable to bind Ku70 in yjr presence of an increasing concentration of s17. (G) Influence of s17 and Ku-55933 on γH2AX accumulation measured 12 h post-transduction with HIV_wt. Data were normalized to DMSO-treated samples. Mean values ± SD (n = 3) are shown in all graphs. Significance was determined with one-way ANOVA with Bonferroni correction, * = adj.p < 0.05, ** = adj.p < 0.01, *** = adj.p < 0.001, **** = adj.p < 0.0001, ns—non-significant.