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Methoxyphenyl and chlorophenyl residues of s17 are essential for HIV-1 inhibition. To measure EC50 of s17 derivatives, 293T cells were transduced with the HIV_wt vector and treated with increasing concentrations of s17 or its derivatives. At 24 h post-transduction, the luciferase expression level was measured. Normalized to DMSO-treated cells; data of three independent repeats were used to calculate EC50.
