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. 1998 Sep;180(17):4360–4369. doi: 10.1128/jb.180.17.4360-4369.1998

FIG. 6.

FIG. 6

Total DNAs of RR22 derivative strains obtained after growth on LB medium, digested with SwaI, and separated by PFGE. (A) Gel stained with ethidium bromide. (B) Southern hybridization with a probe for the clc genes; the probe was a 4.2-kb BglII fragment containing the clcABD genes from P. putida(pAC27) (9). Lanes: 2 to 11, strains RR2231, RR2232, RR2233, RR2234, RR2235, RR2236, RR2237, RR2238, RR2239, and RR2240, respectively; 12, P. putida RR22 after 100 generations on LB medium; 13, RR22 grown on MCB; 14, P. putida F1; 1 and 15, S. cerevisiae molecular size marker (225 to 2,200 kb; Bio-Rad). (C) Copy numbers and genetic organization of integrated clc elements on the two (originally) 400- and 750-kb SwaI fragments in some of the F1 transconjugant strains. The total size of each resulting SwaI fragment is indicated. No absolute location of the clc element integration on the 750-kb SwaI fragment is given.