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. 2023 Dec 15;24(24):17501. doi: 10.3390/ijms242417501

Figure 6.

Figure 6

SAA reduces neutrophil accumulation in lungs during sepsis and attenuates neutrophil transmigration in vitro. (A) Average neutrophil counts/10 frames (40×) in H&E stained lung tissue sections in WT and SAA-TKO mice, 24 h following CS challenge. Data are mean ± SEM from 4–6 female mice/group. (B) Myeloperoxidase activity in lung tissues of WT and SAA-TKO mice 24 h following CLP as described under “Section 4”. Data are mean ± SEM from 4 mice/sex/group. (C) A neutrophil transmigration assay was performed as described under “Section 4”. HL-60 cells were incubated with either PBS (control), SAA (5 μg/mL), HDL (14 μg/mL) or SAA + HDL (1:2.8 ratio w/w) for 1 h at 37 °C before adding to laminin-coated transwells. Cells that migrated to the lower chamber containing 200 nM fMLP were counted and expressed as the percentage of the total cells added. n = 3/treatment. * = p ≤ 0.05, ** = p ≤ 0.01 and *** = p ≤ 0.001.