Figure 1.

Values of the pulse parameters for the biphasic pulse protocols included in the study. All protocols have equal total on-time, i.e., N × 2T_p = 1 ms. T_p—pulse width (equal for positive and negative phase); N—number of pulses; d₁—interphase delay; d₂—interpulse delay. (a) Scenario one: short d₁ and long d₂ (protocols 1, 3, 3′ and 5); (b) scenario two: long d₁ and short d₂ (protocols 2, 4, 4′ and 6). Additionally, protocols 7 and 8 (with equal d₁ and d₂) were added for comparison and tested in the ex vivo study.