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Mass spectrometry parameters of MRM in 5mC and C.
| Compound | Parent (m/z) | Daughters (m/z) | Cone Voltage (V) | Collision Energy (V) | Comments |
|---|---|---|---|---|---|
| Cytidine (C) | 244.23 | 112.06 | 28 | 10 | Quantitative ion pair |
| 244.23 | 94.96 | 28 | 46 | Qualitative ion pair | |
| 5mC | 258.23 | 126.11 | 22 | 10 | Quantitative ion pair |
| 258.23 | 108.89 | 22 | 42 | Qualitative ion pair | |
| Thymidine (IS) | 243.23 | 127.11 | 16 | 10 | Quantitative ion pair |
| 243.23 | 117.06 | 16 | 6 | Qualitative ion pair |
