Table 4.
Antibacterial assay relative inhibition zone diameter (RIZD) results (%).
| RIZD (%) | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gram+ | Gram− | |||||||||||||||||
| S. aureus (ATCC 23235) | S. aureus (C511) | S. aureus (C612) |
E. faecium (C1) |
E. faecium (C14) |
E. coli (ATCC 25922) |
A. hydrophila (C2GSPA1) |
P. aeruginosa (C3GSPR1) |
V. fluvialis (RimA1TCBS) | ||||||||||
| Extracts | SMS | S + CN | SMS | S + CN | SMS | S + CN | SMS | S + CN | SMS | S + CN | SMS | S + CN | SMS | S + CN | SMS | S + CN | SMS | S + CN |
| w-SE | 0 | 88.9 | 0 | 86.7 | 0 | 97.1 | 0 | 88.2 | 0 | 93.8 | 0 | 0 | 44.4 | 88.9 | 66.7 | 75.0 | 30.4 | 89.1 |
| et-SE | 38.9 | 94.4 | 0 | 86.7 | 0 | 91.2 | 0 | 88.2 | 0 | 93.8 | 0 | 87.5 | 0 | 88.9 | 75.0 | 75.0 | 0 | 87.0 |
| w-LE | 0 | 88.9 | 0 | 93.3 | 0 | 97.1 | 0 | 94.1 | 0 | 100.0 | 0 | 0 | 50.0 | 94.4 | 75.0 | 83.3 | 30.4 | 87.0 |
| et-LE | 38.9 | 94.4 | 0 | 86.7 | 0 | 97.1 | 0 | 97.1 | 0 | 103.1 | 0 | 87.5 | 44.4 | 83.3 | 66.7 | 75.0 | 0 | 91.3 |
| w-LE-HT | 0 | 94.4 | 0 | 100.0 | 0 | 100.0 | 0 | 94.1 | 0 | 100.0 | 0 | 43.8 | 50.0 | 100.0 | 75.0 | 83.3 | 0 | 87.0 |
| et-LE-HT | 38.9 | 88.9 | 0 | 93.3 | 0 | 97.1 | 0 | 94.1 | 0 | 100.0 | 0 | 75.0 | 55.6 | 77.8 | 83.3 | 108.3 | 34.8 | 87.0 |
| w-UE | 38.9 | 94.4 | 0 | 93.3 | 0 | 97.1 | 0 | 97.1 | 0 | 100.0 | 0 | 100.0 | 44.4 | 88.9 | 66.7 | 75.0 | 30.4 | 87.0 |
| et-UE | 0 | 94.4 | 0 | 86.7 | 0 | 94.1 | 0 | 100.0 | 0 | 106.3 | 0 | 93.8 | 0 | 83.3 | 75.0 | 75.0 | 0 | 87.0 |
| w-COMB | 0 | 88.9 | 0 | 93.3 | 0 | 97.1 | 0 | 97.1 | 0 | 103.1 | 0 | 81.3 | 38.9 | 88.9 | 58.3 | 75.0 | 0 | 82.6 |
| et-COMB | 0 | 94.4 | 0 | 86.7 | 0 | 94.1 | 0 | 94.1 | 0 | 100.0 | 0 | 75.0 | 0 | 83.3 | 66.7 | 75.0 | 0 | 87.0 |
| w-COMB-HT | 0 | 94.4 | 0 | 93.3 | 0 | 97.1 | 0 | 97.1 | 0 | 103.1 | 0 | 81.3 | 44.4 | 88.9 | 66.7 | 75.0 | 30.4 | 87.0 |
| et-COMB-HT | 38.9 | 100.0 | 0 | 100.0 | 0 | 97.1 | 0 | 102.9 | 0 | 109.4 | 0 | 56.3 | 38.9 | 88.9 | 66.7 | 100.0 | 66.7 | 75.0 |
SMS—spent mushroom substrate; S + CN—extract combined with gentamicin of 10 μg; w—distilled water; et—ethanol 50% (v/v) solvent; SE—short extraction; LE—24 h extraction; LE-HT—24 h extraction with high temperature; UE—ultrasound extraction; COMB—combined methods (LE + UE); COMB-HT—combined methods (LE-HT + UE). The results presented are the mean of replicas (n = 2). The values under SMS represent the discs with mushroom substrate extract. The antibacterial effects of the extracts assessed were classified according to the following activity score: 0%—without effect; 0–<100%—less effective than an antibiotic; >100%—more effective than an antibiotic [41].