Table 1.
Breast cancer cell line classification.
| Cell Line | 1 ER | PR | HER2 | Type | Protein Status | TP53 Status | Additional Mutated Genes | Medium | Morphology | References |
|---|---|---|---|---|---|---|---|---|---|---|
| ZR-75-1 | + | +/− | − | Luminal A | Low Ki-67 | Wild type | PTEN | RPMI | Grape-like | [16,20,21,22,23,24,25] |
| T-47D | + | + | − | Luminal A | Low Ki-67 | L194F | PIK3CA, SPEN | RPMI, DMEM | Mass | [16,20,22,24,25] |
| MCF7 | + | + | − | Luminal A | Low Ki-67 | Wild type | PIK3CA | RPMI, DMEM | Mass | [16,20,21,22,23,24,25] |
| MDA-MB-415 | + | +/− | − | Luminal A | Low Ki-67 | Y236C | MAP2K4, PTEN | DMEM | Round | [7,16,20,22,24,25] |
| MDA-MB-330 | +/− | − | + | Luminal B | High Ki-67 | Y220C | CTNNA1 | RPMI | Grape-like | [7,16,22,25,26,27] |
| ZR-75-30 | + | − | + | Luminal B | High Ki-67 | Wild type | BRCA2, AKT1 | RPMI | Grape-like | [16,25,28,29] |
| BT-474 | +/− | + | + | Luminal HER2 | High Ki-67 | E285K | PIK3CA, BRCA2 | RPMI | Mass | [16,20,21,22,24,25] |
| MDA-MB-361 | + | +/− | + | Luminal HER2 | High Ki-67 | E56 * | PIK3CA, SPEN | RPMI, DMEM | Grape-like | [7,16,22,24,25,30] |
| UACC-812 | +/− | +/− | + | Luminal HER2 | High Ki-67 | Wild type | BRCA1 | L-15, RPMI, DMEM | Grape-like | [16,24,25,30,31,32,33] |
| AU565 | − | − | + | HER2+ | High Ki-67 | R175H | CDH1 | RPMI | Grape-like | [16,21,24,25,30] |
| MDA-MB-453 | − | − | + | HER2+ | High Ki-67 | T382S | BRCA2, PTEN, PIK3CA | RPMI, DMEM | Grape-like | [7,16,20,21,24,25] |
| HCC1569 | − | − | + | HER2+ | High Ki-67 | E294 * | PTEN, PIK3CA | RPMI | Mass | [20,24,25,34,35] |
| SUM190PT | − | − | + | HER2+ | High Ki-67 | Q317 * | PIK3CA | Ham’s F12 | Mass | [2,12,16,25,36] |
| SK-BR-3 | − | − | + | HER2+ | High Ki-67 | R175H | CDK2NA | DMEM, McCoys | Grape-like | [16,20,21,24,25] |
| HCC70 | − | − | − | TNBCA | High Ki-67 | R248Q | FANCA, PIK3CA | RPMI | Mass | [16,20,24,25] |
| BT-20 | − | − | − | TNBCA | High Ki-67 | K132Q | RB1, PIK3CA | EMEM, RPMI, DMEM | Stellate | [16,20,21,25,37,38] |
| CAL148 | − | − | − | TNBCA | High Ki-67 | E224K | RB1, PTEN, PIK3CA | DMEM | Round | [16,25,30,38] |
| SUM229PE | − | − | − | TNBCA | High Ki-67 | R273C | CDK2NA, PIK3CA | RPMI, Ham’s F12 | Spindle | [2,16,25,30,37,39] |
| BT-549 | − | − | − | TNBCB | High Ki-67 | R249S | RB1, PTEN | RPMI | Stellate | [16,20,24,25] |
| MDA-MB-157 | − | − | − | TNBCB | High Ki-67 | A88fs | MED12, SPEN | RPMI, DMEM | Stellate | [7,16,20,25,40] |
| MDA-MB-231 | − | − | − | TNBCB | High Ki-67 | R280K | BRAF, TERT, KRAS | RPMI, DMEM | Stellate | [7,16,20,21,24,25] |
| Hs 578T | − | − | − | TNBCB | High Ki-67 | V157F | PIK3CA, MED12, CDKN2A | RPMI, DMEM | Stellate | [16,24,25,30] |
| MDA-MB-435 | − | − | − | MEL | High Ki-67 | G266E | BRAF, CDKN2A | L15, RPMI, DMEM | Spindle | [16,20,25] |
1 ER (estrogen receptor), PR (progesterone receptor), HER2 (human epidermal growth factor receptor 2), and MEL (Melanoma). Medium is the culture media that the breast cancer cells can be grown in, but this is not limited to what is shown. Options for media include EMEM (Eagle’s Minimum Essential Medium), L-15 (Leibovitz’s L-15 Medium), RPMI (Roswell Park Memorial Institute-1640 Medium), DMEM (Dulbecco’s Modified Eagle Medium), McCoys (McCoys 5a Medium) and Ham’s F12 (Ham’s F-12 (Kaighn’s) Medium). All media have nutrients added to ensure proper cell viability (see Section 3.3.1 for more information). +/− represents the positive or negative receptor that the breast cancer cell line is classified to have The type consists of the receptors that are present or not present within the breast cancer cell line such as Luminal A, Luminal B, Luminal HER2, HER2+, and Triple-Negative Breast Cancer (TNBC) that can be further divided into Basal-like (TNBCA) and Normal-like (TNBCB). Protein status consists of the level of Ki-67 proliferation index of the breast cancer cell line type. TP53 status indicates the protein sequence mutation for each breast cancer cell line listed. Some of the amino acid alterations have an asterisk symbol “*” that indicates a premature stop codon that is produced from the TP53 mutation. Wild type indicates that the breast cancer cell line does not have a mutation within the TP53 gene. Morphology consists of stellate, mass, grape-like, spindle, and round.