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. 2023 Nov 29;21(12):624. doi: 10.3390/md21120624

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Reduction of BODIPY-cholesterol uptake by laminarin in Caco-2 cells. (A) The viability of Caco-2 cells was assessed using the Resazurin Conversion Assay (n = 3). (B–D) Caco-2 cells were treated with different concentrations of laminarin (10, 25, 50, and 100 μM) and ezetimibe (50 μM), and the uptake of BODIPY-cholesterol was evaluated. Panel (B) shows confocal images of the cells (magnification at 63×; Scale bar, 50 μm), and Panel (C) shows the quantitative analysis of fluorescence intensity (n = 3). (D) BODIPY-cholesterol uptake in Caco-2 cells was measured by Flexstation 3 (n = 3). All data are expressed as the mean ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, n.s., no significance (one-way ANOVA).