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. 2023 Nov 29;21(12):624. doi: 10.3390/md21120624

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Silencing NPC1L1 attenuates the inhibitory effect of laminarin on cholesterol uptake in Caco-2 cells. (A) Quantification and representative Western blot of NPC1L1 expression in Caco-2 cells after a 72 h knockdown of NPC1L1 using different siRNAs (n = 4). (B–D) BODIPY-cholesterol uptake in wild-type and NPC1L1 knockdown Caco-2 cells treated with 50 μM laminarin and 50 μM ezetimibe or a combination of both. Confocal images of the cells are shown in Panel B (magnification at 63×; Scale bar, 50 μm), and Panel C presents the analysis of fluorescence intensity (n = 3). BODIPY-cholesterol uptake determined by Flexstation3 is shown in Panel D (n = 3). Values are presented as mean ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001, n.s., indicates no significance (one-way ANOVA).