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Purification of Nir from S. thioluteus
| Purification step | Total protein (mg) | Total activity of (μmol of NO/min) | Sp act (μmol of NO/min/mg) | Recovery (%) |
|---|---|---|---|---|
| Solubilized extracts | 4,218 | 3,260 | 0.76 | 100 |
| CM-cellulose | 49 | 2,320 | 47 | 71 |
| Gel filtration | 8.6 | 1,104 | 128 | 33 |
