TABLE 1.

PDEA and DGC activities and in vivo cellulose production in A. xylinum recombinant strains^a

Strain	Site of mutation	PDEA activity (%)	DGC activity (%)	Cellulose production (%)
1306-21 (WT)	None	100	100	100
Dis1	pdeA1	20	20	85
Dis4	dgc1	98	21	80
ABT8	Upstream of cdg1a	23	22	78
ABT9	pdeA2	85	85	98
TRT150	dgc2	98	86	95
ABT2	Partial deletion (pdeA2+dgc2)	89	91	99
ABT21	dgc1 dgc2	105	4	36
ABT11	pdeA1 pdeA2	5	5	68
ABT1	pdeA1 pdeA2 dgc2	5	4	70

^aRecombinant strains of A. xylinum disrupted in cdg1 and/or cdg2 were derived, and whole cells were assayed for cellulose production, as described in Material and Methods. Soluble and membrane fractions were prepared from logarithmic-phase cultures and assayed for PDEA and DGC activities.